Vegetative propagation
This is the natural regeneration of plants other than seeds
to develop new plants. The new plants
are sometimes called Clones
Advantages of vegetative propagation
- It
is possible to maintain desirable characteristics such as disease resistance
and physical appearance
- It
makes it possible to propagate crops that are seedless or have viable
seeds
- The
growth habit of a crop can be regulated to attain a desirable shape and
size
- It
is possible to produce more than one crop variety on the same plant
- Vegetative
propagation imparts desirable characteristics to crops, such as seedless
in citrus fruits
- It
is completely independent of the processes of pollination and fertilization
- It
enables cross-bred plants to be maintained in their new condition indefinitely
- The
new plants are exactly the same as
the parent plant when vegetatively propagated
- The
young plants are hardy and vigorous than seedlings. This is due to a lot of food reserves
available to the growing plant
- Vegetatively
propagated plants grow and mature faster than seed propagated plants
Disadvantages of vegetative propagation
- Vegetatively
propagated plants are difficult to breed and genetically improve since
there is no possibility for cross breeding
- If
the vegetative materials curry a disease there is the danger of
transferring diseases to the new plants or locations
- Vegetative materials are bulky , making them
difficult to transport and plant out
- Due
to high moisture content, most vegetative materials cannot be stored for a
long time, they lose viability or sprout in store
- It
is difficult to dress vegetative materials against diseases and pests
- Vegetative
materials' planting cannot easily be mechanized
- Due
to the amount of materials required and the transport costs involved. It is expensive to plant a large field
with vegetative materials
Methods of vegetative propagation
These involve use of specialized vegetative and perennating
organs
Bulbs; a bulb is modified shoot with short stem and
storage leaves. Bulbs are used in
propagation of onions, garlic, and tulips.
Corms; a corm is a short, swollen, vertical underground stem. Corms are used in propagation of coco yams,
taro
Stolons; this is a creeping horizontally growing stem
that grows on top of the soil surface. It
is used in propagation of blackberry, gooseberry, blackcurrant, and redcurrant
Runners; runners bear axillary buds and the buds give
rise to adventitious roots and new plants.
They are used in propagation of strawberry and creeping buttercup
Rhizomes; rhizomes bear leaves, buds and adventitious
roots. Rhizomes are used in propagation
of ginger, cardamom, and turmeric.
Suckers; these shoots which radiate from buds below
the ground level. They are used in
propagating crops such as banana, pineapples, and sisal
Tubers; tubers have axillary buds that have capacity
to sprout in to new independent plant. Tubers
are used in propagation of crops like; potatoes and arrow root and yams
Bulbils; these are aerial bulbs or buds produced in
leaf axils, which on separation, are capable of growing into a new plant. They are used in propagating sisal.
Turf splits; turf is a clump or bunch of tillers
originating from a common base. Turf
splits are used in propagation of most pasture grasses
Crowns; these develop from the top of fruits. Mature crowns on fruits are dormant but
become active after detachment from the fruit.
They are used in propagating pineapples
Slips; these are shoots borne on the peduncle just
below or on the base of the fruit. Slips
are used in propagation of pineapples.
Vines; these are soft cuttings obtained from mother
plants and planted directly into the main field to give rise to new plants. They are used in propagation of sweet
potatoes.
Cuttings
These are pieces of plants (stem, roots, or leaves) that are
cut and then planted. They have buds
which develop into a shoot. Cuttings are
used to propagate plants like sugarcane, tea, and cassava.
The ability of the cuttings to root and shoot depends on the
following factors;
- Plant
species; some plant species have natural ability to establish from
cuttings. These cuttings easily
sprout when provided with suitable growth medium
- Age
of the plant; cuttings from young plant parts root better and easily than
leaf or root cuttings.
- Type
of rooting medium; the rooting medium
must be sterile , porous but capable of providing sufficient oxygen and
moisture
- Chemical
and other treatments given to cuttings such treatment with growth hormones
- Weather
conditions such as humidity , temperature and sunlight
- Watering
frequency
- The
amount of leaves on the cutting
Cuttings to use for plant propagation should have the
following qualities
- Should
have reasonable number of buds and
the buds should be viable
- Should
be free from pest and diseases
- Buds
should be free from mechanical or pest or diseases damage
- Should
be of average maturity with enough food reserves
- Should
be from desired variety
- Should
be alive or fresh for optimum viability
- For
some cuttings, should have optimum number of leaves
Layering
Layering is the practice of stimulating a part of a branch
to produce roots while still attached to the mother plant. The rooted part is then cut off and planted
as a new plant
Procedure of layering
- The
process of layering starts with selection of a suitable branch on the
growing plant, the branch should not be having any form of deformities and should be
free from pests or diseases
- The
selected branch is then wounded mildly.
Wounding allows transportation and accumulation of healing and
growth hormone around the wound. Accumulation
of especially Idole acetic acid (IAA) induces growth of roots around the
wound. The process of rooting is
also encouraged by presence of suitable rooting medium around the wound
- Prepare
the rooting medium; the rooting medium can be sandy soil mixed with
organic matter or peat soils. The
rooting medium should be well sterilized, provide optimum aeration and
moisture.
- The
rooting medium is then placed around
the wound and then wrapped with a piece of perforated polythene
sheet or cloth
- The
rooting medium should be watered periodically to prevent drying of the
rooting medium
- After
about 2-4 months depending on the
species, when the roots have fully developed, the branch is cut off and
then planted out in pot nursery or directly planted in the field
Types of layering
Marcotting or aerial layering; it is used when the
branch or the stem cannot bend to touch the ground; instead, the soil is
curried up and tied to the branch. This
form of layering requires more skills and labour, and success is fairly low.
Tip layering; in this method, the tip of the shoot
bearing the terminal bud is wounded and then bent to the ground. It is then held in position using pegs and
covered with layer of moist soil. The
covered part is induced to produce roots by applying a moist rooting medium
Compound or serpentine layering; in this method, the
branch is bent several times and held in the soil with pegs. This produces several new rooted plants from
the mother plant. This form of layering
is commonly used in runners and stolons
Trench or ground layering; in this method, the branch
to be layered is first wounded, then bent to the ground and laid in a trench. It is then held in position using pegs and covered
with a layer of moist soil. The covered
part is induced to produce roots by applying a moist rooting medium. The branch is then cut off from the mother
plant and transplanted
Stool or mound layering; this method is used when the
branch or stem cannot bend enough to reach the ground level. In this form the soil the soil is heaped high
to form a mound where the branch or stem can be able to reach or the rooting
medium is placed in the container and then raised high supported on a stand or
stool, and then the branch is slightly bent into the rooting medium in the
container.
Benefits of layering
- The
propagule has high chances of survival than cuttings
- A
large planting material is obtained in a short period of time
- Plants
propagated from layered propagules mature faster than plants from cuttings
- Seedlings
from layering are more adapted to the environmental conditions
- Seedlings
from layering are more resistant to pests and diseases
Disadvantages of layering
- The
method requires more skills to curry out
- There
are high chances of spreading pests and diseases from mother plant to the
propagules
- Propagules
are usually large requiring more labour to handle and plant
Grafting
This is an artificial plant propagation method involving
uniting a shoot from one plant to the root stock or another plant so that they
grow as one plant. The shoot and the
rootstock should be from plants of the same species.
The upper portion of the graft is called the scion whereas
the lower portion of the graft is called the stock. Grafting is only done in woody plants and in
plants of the same species.
The union of the graft is due to regeneration of cambial
tissues; callus cells are responsible for the healing of the union wound.
Advantages / benefits of grafting
- Grafting
can be used to change the plant from being undesirable to desirable. This is done by replacing the undesired
shoot with desired shoot
- It
enables propagation of crop that would be difficult to propagate by other
means
- Grafted
crops mature faster than seed propagated crops
- Grafting
can be used to test for disease resistance in some crops. This is done by grafting susceptible
scion to a resistant stock and observing the response
- It
makes the growing of more than one type of plant on one stock possible. This economizes space required to grow
the different types of crops in the garden
- Root
stocks with desirable qualities such as disease and pest resistance,
drought resistance and resistance to salinity can be harnessed for
production of beneficial but susceptible crops
- Grafting
may be used to repair damaged plant parts; when the stem or branch is
damaged either by pests or browsing animal, it can be cut off and replaced
with a better health stem or branch
- Provides
an opportunity of utilizing good qualities of the rootstock and scion of
different plants
Disadvantages of grafting as mean of propagation of crops
- The
method requires a lot of skills to carry out
- It
is not reliable as any slight mistake can lead to total failure.
- It
can only be done in propagation of some few crops
- The
method requires a lot of time and labour
- Grafted
crops can easily be damaged by wind
- Spread
of diseases from the mother plant to the grafted plants may occur
- Grafting
can only take place in closely related families thus not giving a chance
to have different families on the same root stock.
Procedures of grafting
- Select
a seed from a mother that is vigorous and well adapted to the soil
condition to provide the rootstock
- Raise
the seed in a pot nursery
- A
seedling is ready for grafting when the stem is pencil thick
- Select
a dormant shoot from a mother plant with desired qualities and of the same
species as the rootstock to provide the scion
- Grafting
should be done on a cool day or in a cool environment
- Use
a sterilized razor blade or grating blade to cut the scion and the stock.
- Make
matching cuts on both the scion and the stock. The cuts can be V-shaped or straight
slanting cut on both the scion and stock.
- Remove
all the leaves from the scion to reduce transpiration
- Join
the scion to the stock; make sure the cambia of the scion and the stock
are in contact
- Tie
the joint with grafting tape and apply grafting wax to prevent bacterial
and fungal infection
- Keep
the grafted plant in cool humid environment and away from wind until buds
starts to grow
- Remove
the grafting tape after the joint has healed and then transplant to the
main field
Conditions that should be fulfilled for grafting to be
successful
- The
scion and the stock must be from woody plants
- The
scion and the rootstock must be from plants of the same species
- The
scion and the rootstock must be of the same growth habit
- The
scion and the rootstock should preferably be of the same diameter
- During
grafting the cambia of the scion and the rootstock must be in contact
- The
scion must be dormant at the time of grafting
- The
scion and the rootstock must be at the proper physiological stage of
growth
- Post
graft care such as keeping the graft union moist and protected from
infection until the wound has healed
Factors affecting the success of grafting
- Compatibility
of the scion and the root; the scion and the rootstock should be from
genetically related plants (plants of the same species)
- Type
of the plant; grafting is only possible in some plants especially those
with woody stems
- Grafting
technique used; the cuts made in the scion and rootstock should be matching
for the joint to be firm and to encourage maximum cambial contact
- Post
grafting care; good care of newly grafted plants is vital to accelerate
union and healing of the plants. Plants
should be provided with warm temperature and high humidity and the joint
protected from infection.
- Pest
and disease infestation; any pest or disease infestation to the newly
grafted plants can lead to failure of the plants. These can be controlled by ensuring high
level of hygiene in the nursery.
- The
quality of the rootstock or the scion; these should be healthy and
vigorous. The scion should be
dormant and not actively growing at the time of grafting.
Incompatibility in grafting
Graft incompatibility is a situation where the grafted
plants fail grow as one plant exhibited by death of the scion
Signs of incompatibility in grafted plants
- Browning
of the scion
- Premature
death of the planting Abnormal growth of the scion or rootstock
- Poor health of the plant
Causes of graft incompatibility
- Physiological
and bio chemical differences between the scion and the rootstock
- Grafting
of plants of different growth habits
- Diseases
especially viral infections to scion
- Incompetent
workmanship (lack of grafting skills)
- Unfavorable
micro-climate provided to the grafted planting
- Grafting
of actively growing scion
Methods of grafting
Splice grafting; a straight slanting cut is made on
both the scion and the stock so that the joining surfaces come together
obliquely
Cleft grafting; the top of the stock is cut off and a
wedge cut is made in the rootstock where a wedge shaped scion into the stock
Saddle grafting; a V-shaped cut is made on the stock
and the scion is cut in wedge shape that fits properly into the stock
Whip or tongue grafting; in this method , a straight
slanting cut is made on both the scion and the stock and then after a second
cut is made on both scion and stock to produce a notch -like arrangements that
accommodates each other and fit slightly together
Side grafting; in this method V-cut is made on the
side of the rootstock at an angle of about 30 where a wedge shaped scion is
fitted
Budding or bud grafting
This form of grafting which involves uniting a bud from one
plant to the rootstock of another plant where it regenerates as new plant. The bud and the scion should be from plants
of the same species.
The bud to graft should be in dormant state while the stock
should be the one that is highly adapted to soil condition and vigorous.
Methods of budding
T-budding
It is used on young socks with a bark which is able to slip
off. In this method a vertical cut is
made through the bark of the rootstock and after, a horizontal cut is made at
the top of the vertical cut to make a T-shaped cut. The bark is then loosened and the bark flaps lifted. A shield shaped piece of bud is inserted
under the bark and the union is wrapped with the grafting tape
Patch budding
This is used on old stocks with hard bark or with a thick
bark that cannot peel off or cannot be lifted from the wood. In this method a rectangular piece of bark is
removed from the rootstock where a rectangular shaped bud is inserted and then
wrapped with grafting tape
Procedure for bud grafting
- Select
a seed from a mother that is vigorous and well adapted to the soil
condition to provide the rootstock
- Raise
the seed in a pot nursery
- A
seedling is ready for bud grafting when the stem is pencil thick
- Bud
grafting should be done on a cool day or in a cool environment
- Select
a dormant bud from a plant of the same species as the rootstock
- Use
a sterilized razor blade or grafting blade to cu the bud. Peel off wood from the bark of the bud
patch
- Make
a T-cut in the bark of the rootstock and gently lift up the bark
- Gently
push the bud under the bark
- Wrap
the bud with grafting tape and apply grafting wax to prevent water and infection from
bacteria and fungi
- Cut
off the part of the stock above the bud and remove any other bud from the
rootstock
- Inspect
the bud after about 15- 20 days, green color of the bud indicates that the
bud is still alive and that the graft is successful. Browning of the bud indicates that the
bud is dead
- Buds
are ready for transplanting after 40- 60 days
Care given to drafts and buds
- Wrapping
the grafts and buds with grafting tape to make the union firm and to
prevent the scion from falling off.
- Protection
of the union against water that can lead to rotting
- Reducing
the amount of leaves to reduce transpiration and dehydration of the scion
- After
the union is firm, the grafting tape should be removed to prevent girdling
due to buildup of translocate substance above the union
- Once
the bud is established the part of the rootstock above the bud should be
cut off to encourage the bud to sprout
Advantages of budding
- Budding
is simple than grafting
- Shoots
from buds are more strong and resistant to wind damage than shoots from
grafts
- Budding
is more economical since a single shoot can produce several buds
- Several
buds from different varieties can be grafted on the same stock easily
- Unlike
in shoot grafting buds do not easily spread pests
Tissue culture
Tissue culture is the growing of living cells from shoot
tips in a controlled medium. In tissue
culture, living tissues from desired varieties are placed in the growth medium
that leads to rapid cell division to take place. The cells are then cloned (sub culture) each
cell developing into independent plantlet.
The culture medium is made up of nutrients, indoleacetic acid, and
cytokinins
Advantages of tissue culture as a method of propagating
crops
- Tissue
culture seedlings are free from diseases
- Many
seedlings can be obtained in a short period of time
- The
seedling are not as bulky as cuttings
- In
crops which are difficult to produce using seeds or vegetative organs,
this is the only quick means of multiplying such crops
- Crops
are identical genetically and all crop are the same in behavior e.g
uniform ripening of crops making harvesting easier
Limitations of tissue culture as means of propagating crops
- The
method is expensive since it requires special lab equipment and chemicals
- The
method requires special skills to carry outbreak
- Cell
mutation may take place it the lab resulting into change in crop behaviour
- Tissue
culture seedling take more time to mature
- In
some crops such as root and tuber crops the first harvest is always low
- The
seedlings are highly susceptible to pests and diseases